Date: 25 January 2023, 15:00-17:30
Location: Online at Zoom (link will follow in an e-mail after registration deadline)
Organiser: DCCC Danish Research Centre for circulating tumor DNA guided treatment
Registration: Please register with this link
Registration deadline: 20 January 2023
Contact: Anne Lærke Lorentzen, e-mail: anne.lorentzen@clin.au.dk
The ctDNA seminars will be held approximately every second month.
They are open for everyone to participate in - so feel free to share it with others who are working with or interested in ctDNA
The seminar series is an element of the ctDNA Research Center’s aim to facilitate national collaboration regarding research on ctDNA guided cancer treatment.
The 1st ctDNA seminar will be about digital PCR data handling
Many studies involving circulating tumor DNA (ctDNA) detection are using digital PCR (dPCR) to quantify ctDNA. One of the attractive qualities of dPCR is the relatively simple data output format, enabling easy data processing. Yet, dPCR is – like all molecular tools – susceptible to noise, which can complicate quantification of low ctDNA levels.
This online workshop will introduce basic data handling of dPCR data and via practical exercises guide participants through the steps of correcting for noise in dPCR data. Finally, the workshop will showcase examples of how substandard data handling can lead to problematic study results.
Learning outcomes:
- Defining positive and negative droplets
- Applying different methods to differentiate between noise and signal
- Understanding factors impacting the noise level in cfDNA analysis
- Understanding the requirements for various noise-correction methods
- Discern which noise-correction method is suitable for different studies
- Appreciate the implications of false-positives and false-negatives on a real-life study